Peripheral nerve blocks are commonly used in regional anesthesia. In recent years, it has become a reliable technique owing to the low complication and high success rates of application using ultrasound (US) [1-3]. However, there are certain difficulties such as Pinaccurate evaluation of block success because available evaluation techniques (cold sensation, pinprick, muscle force, etc.) are composed of subjective tests [4,5]. Additionally, a cooperative patient is an essential requirement, as well as anatomic information and experience, for the successful applicability of these tests [4,5]. A rapid, reliable, and noninvasive assessment method is needed to accurately evaluate block success.

It has been shown that sympathetic blockade which develops due to peripheral nerve block causes vasodilatation and increased peripheral blood flow and consequently leads to elevated distal skin temperature [6-8]. Although elevated skin temperature has been demonstrated, there is no absolute conclusion regarding the advantage of skin temperature in evaluating and predicting the success rate of nerve block.

Near-infrared spectroscopy (NIRS) is a novel, continuous, and non-invasive method for monitoring regional tissue oxygenation of cerebral and somatic beds by providing real-time feedback [9]. NIRS, which was initially used for the assessment of cerebral oxygen saturation, is commonly used nowadays to evaluate somatic tissue perfusion [9].

Assuming that sympathetic blockage can affect tissue oxygen saturation due to vasodilation and increased peripheral blood flow, we hypothesized that the evaluation of tissue oxygen saturation determined by NIRS would be a predictor of peripheral nerve block success.

Institutional ethics committee approval (Necmettin Erbakan University Meram School of Medicine, no. 2016/464) and written consent from the patients were obtained for this prospective, observational study. It has been registered in the Australian New Zealand Clinical Trial Registry (ACTRN12616000656437). Forty patients between the ages of 18-65 years, American Society of Anaesthesiologists physical status I and II, scheduled for acute or elective hand or forearm surgery under lateral infraclavicular block, were studied. Exclusion criteria were age < 18 years, morbid obesity defined as a body mass index (BMI) > 40, international normalized ratio > 1.4, platelet count < 80 × 10⁹ L^-1, coagulopathy, medication with vitamin K antagonists, high-dose or fractionated heparin treatment, allergy to local anesthetics, infection at the site of needle insertion, peripheral neurological disease, Raynaud’s phenomenon, and patient refusal. In all patients, a 20-gauge intravenous (IV) cannula was inserted into the radial vein at the wrist of the non-blocked hand for IV fluid and medication administration.

Routine monitoring (consisting of a pulse oximeter, 3-lead electrocardiogram, and non-invasive blood pressure cuff) was performed.

Forty patients were enrolled in the study, and all completed the study. Twenty-seven patients underwent elective surgery, and 13 patients were scheduled emergently. No patient withdrew from the study after the LIC block. Thirty-one of the performed LIC blocks were successful, 5 were partial failures, and 4 were complete failures. In a total of 9 failed blocks (complete and partial), the LIC block was successfully repeated preoperatively. Successful block patients did not require additional intraoperative analgesics, and visual analogue scale scores were 0 for 12 h postoperatively. Patient demographic data are summarized in Table 1. There were no significant differences between the three groups regarding sex, age, weight, height, BMI, and American Society of Anaesthesiologists physical status (P=0.436, P=0.459, P=0.906, P=0.774, P=0.826, and P=0.459, respectively).

The NIRS values from the baseline to 30th min are shown in Fig. 1. Baseline NIRS values were 70.84 ± 6.79, 70.20 ± 6.72, 65.75 ± 3.77, and 69.63 ± 8.14 in successful, partial failure, complete failure patients and contralateral side, respectively, and showed no statistical difference (P = 0.834). NIRS values were significantly higher in the successfully blocked patients than in the complete failure patients and contralateral side (P = 0.003 and P < 0.001, respectively) at the 5th min. However, there was no statistically significant difference between NIRS values of successful block patients (81.94 ± 6.82) and partial failure patients (73.80 ± 6.30) at 5 min (P = 0.164). NIRS values were significantly higher in the successfully blocked patients from the complete failure, partial failure, and contralateral sides (P < 0.001, P = 0.008, and P < 0.001, respectively) at the 10th min. There was no statistically significant difference when comparing NIRS values at all measurement times (5th, 10th, 15th, 20th, 25th, 30th min) between complete failure and partial failure block patients (P=0.996, P=0.467, P=0.373, P=0.321, P=0.183, and P=0.261, respectively).

The changes in the Δ NIRS values are shown in Fig. 2. In the successfully blocked patients, NIRS values (mean ± SD [Change in %]) increased by 11.09 ± 4.86 (16.03%), 15.00 ± 4.53 (21.76%), 16.35 ± 5.14 (23.77%), 16.38 ± 4.88 (23.85%), 16.67 ± 5.04 (24.29%) and 16.96 ± 5.71 (24.78%), respectively, from baseline to 5, 10, 15, 20, 25, and 30 min (P < 0.001). Δ NIRS values were significantly higher in the successfully blocked patients than in the partial failure patients, complete failure patients, and contralateral side (P = 0.047, P = 0.002, and P < 0.001, respectively) at the 5th min. Δ NIRS values were significantly higher in the successfully blocked patients from the complete failure, partial failure, and contralateral groups (P < 0.001, P < 0.001, and P = 0.008, respectively) at the 10th min. NIRS values increased by 21.76% in the successfully blocked patients at 10th min according to baseline, whereas this increase was 8.54% in partial failure patients (P = 0.003). NIRS values increased by 24.78%, 10.83%, 1.14%, and -5.26%, respectively, in the successfully blocked, partial failure, complete failure patients and contralateral, from baseline to 30th min (P < 0.001, P = 0.225, P = 0.996, and P = 0.448).

The Ts values from baseline to 30th min are shown in Fig. 3. Ts increased from 31.77 ± 1.28 to 32.19 ± 1.54, 33.07 ± 1.56, 33.74 ± 1.27, 34.15 ± 1.18, 34.41 ± 1.10 and 34.46 ± 1.20 in the successfully block patients from baseline to 5th, 10th, 15th, 20th, 25th, 30th min (P = 0.054, P < 0.001, P < 0.001, P < 0.001, P < 0.001, and P < 0.001, respectively). In the three patients with completely failed blocks, Ts decreased from baseline to 30th min (-0.9°C, -1.5°C, and -0.9°C). However, in one of the patients with complete failure, block Ts increased from baseline to 30th min (0.6°C). In complete failure block patients, skin temperature and Δ skin temperature did not show statistically significant difference from baseline to 30th min (P = 0.366 and P = 0.252, respectively).

Ts in one of the patients with partial failure decreased from baseline to 30th min (-3.9°C). Ts in the remaining four patients with partial failure block Ts increased from baseline to 30th minute (+3.2°C, +2.1°C, +3.6°C, and +1.9°C). In partial failure block patients, skin temperature and Δ skin temperature did not show satitstically significant difference from baseline to 30th min (P = 0.162 and P = 0.156, respectively).

There were no statistically significant differences when comparing Ts values of complete failure and partial failure block patients at all measurement times (5th, 10th, 15th, 20th, 25th, 30th min) (P = 0.992, P = 0.996, P = 0.588, P = 0.185, P = 0.155, and P = 0.063, respectively).

The changes in ΔTs values are shown in Fig. 4. Δ Ts (baseline to 30 min) values were 2.69 ± 1.40, 2.20 ± 1.39, 1.47 ± 2.89, and -0.43 ± 1.11, in the successful block, partial failure, and complete failure patients and contralateral side (P < 0.001, P = 0.223, P = 0.996, and P = 0.102, respectively). Δ Ts values were significantly higher in the successfully blocked patients than in the complete failure patients and contralateral side (P < 0.001 and P = 0.008, respectively) at the 30th min. However, there was no statistically significant difference when comparing Δ Ts values of the successful block and partial failure block patients at the 30th min (P = 0.162).

To the best of our knowledge, this is the first study to evaluate tissue oxygen saturation changes in the forearm and hand according to NIRS responses after US-guided LIC blocks. The essential purpose of this study was to comprehensively evaluate these changes and assess the efficacy of NIRS in predicting blockade success. We planned to define the amount and timing of the changes in NIRS values for evaluation. Beginning from the 10th min after the LIC block, NIRS values in the successful block patients were significantly different compared with partial failure, complete failure patients, and contralateral hand. In the successful block patients, NIRS values showed an increase of 21.76% at the 10th min compared with baseline values, and this increase continued until the 30th min. Completely failed block occurred in only four patients, while partially failed block occurred in five patients. The NIRS values of the successful block patients increased significantly 30 min after the block compared to both baseline NIRS values and those in the partial failure, complete failure patients, and contralateral hand. However, no significant increase occurred in either the NIRS or Ts values of the partial failure patients compared with the complete failure patients.

The other essential purpose of the present study was to define changes in Ts after a successful LIC block. The change in Ts values at 30th min was significantly higher in the successful block patients than in the complete failure patients and the contralateral hand, whereas no significant difference was found compared with partial failure patients.

Local anesthetic agents should be able to block both sensory and sympathetic nerve fibers for a successful block [13]. The clinical control of sensory fibers is evaluated using methods such as cold response and pinprick sensation [13-15]. The blockade of small non-myelinated sympathetic nerve fibers using local anesthetics causes vasodilatation, increased blood flow, and increased local temperature. It is known that sympathetic nerve fibers run peripherally along both major nerves and vessels [16]. In the present study, somatosensors were placed on the ventral aspect of the midforearm. We assumed that this region is the most intensive region for major vascular structures of the forearm and thereby would be the most appropriate site for the evaluation of tissue oxygen saturation.

Changes in Ts and thermography values after upper-extremity peripheral nerve blocks have been evaluated in many studies. Minville et al. [8] evaluated skin temperature after nerve stimulator-guided infraclavicular nerve block. They concluded that the block was successful only if nerve-distribution density increased in four major nerves (musculocutaneous, radial, median, and ulnar nerves). On the other hand, it has been stated in subsequent studies that peripheral blockade of the ulnar and median nerves leads to characteristic increases in hand-skin temperature, whereas peripheral blockade of the radial and musculocutaneous nerves caused no change in hand-skin temperature [12]. The palmar face of the fourth digit which represents a thermographically overlapping area between the median and ulnar nerves, appears to be an ideal area to rapidly and accurately assess the change in distal Ts value [17]. However, the intersection of the median and ulnar nerves makes this area diagnostically insignificant. Therefore, the first, second, and fifth digits are commonly preferred for the evaluation of distal Ts [12,17,18]. On the other hand, in the present study, skin temperature could not be measured at these sites because most of the patients would undergo hand surgery and their digits would be injured and bandaged or casted. Consequently, the skin temperature was measured at the bilateral wrist foldings, which is described in the literature as one of the sites used to evaluate skin temperature after upper extremity peripheral nerve blocks [18].

Asghar et al. [18] have preferred different anatomical regions to evaluate the efficacy of distal infrared thermography and skin temperature in predicting the success of US-guided interscalene brachial plexus in their study. The first, second, and fifth digits were used to evaluate the distal aspect of the hand. The wrist region of the hand was also used in a manner similar to that in our study. They found that skin temperature increased by 3.1°C in the wrists of successful block patients (from baseline to 30th min), whereas our study showed an increase of 2.69°C. Further, it has been noted in this study that the distal Ts of the thumb would be the most appropriate region for the prediction of block success.

Asghar et al. [17] evaluated the change in distal Ts from the second and fifth digits of patients who underwent infraclavicular block in another study and detected a significant elevation at 30th min compared with baseline values in successful block patients. Similar to our results, they stated that no significant change was observed in patients who received the failed block. However, we found no significant difference between patients with successful block and those with partially failed block in terms of the values at the 30th min. We believe that this may be due to the measurement site of Ts used in our study. Additionally, the distal skin temperature is exposed to great fluctuations due to changes in environmental temperature, sympathetic nerve activity (stress, anxiety), local inflammation, and previous trauma. We tried to cool both hands by putting them on cold packages to reduce the effects of these factors and standardize the measurements. However, fluctuations may have occurred because of the procedure itself, as well as the reasons mentioned above.

Thermography studies have become prominent in the successful evaluation of peripheral nerve blocks as well as the measurement of distal skin temperature. It is known that sympathetic nerve fibers run peripherally along both major nerves and vessels [16]. Hence, it may be difficult to predict thermographic patterns at various levels of brachial plexus block, such as axillary brachial plexus block, lateral infraclavicular block, and interscalene brachial plexus block.

Besides the present study, there is still insufficient data on evaluation of the changes in the tissue oxygen saturation based on NIRS responses and efficacy assessment of NIRS in the prediction of block success, whereas the number of studies on distal skin temperature and thermographic assessments to evaluate the success of peripheral nerve blocks have progressively increased. Nerve block was successfully evaluated using NIRS after blockade of the stellate ganglion [19], and it was stated that the use of NIRS would be appropriate to evaluate the success of the nerve block [19,20]. Tighe et al. [11] evaluated tissue oxygen saturation using NIRS after peripheral nerve blocks; however, they demonstrated no significant efficacy. However, these outcomes may be due to the use of a small number of patients, as they have stated, along with the application of various blocks in an identical methodology. In contrast with this outcome, NIRS appears to be an effective method for the prediction of successful block in our study compared with both completely and partially failed blocks beginning from the 10th min following the nerve block procedure. In addition, its practicality and clinical understanding are easier to comprehend than those of thermographic measurements.

In other studies, pulse oximetry and oxygen electrodes following a peripheral nerve block were used to detect changes in tissue oxygenation and perfusion [21-23]. However, none of these methods include non-invasive measurements of the subcutaneous oxygenation state following upper and lower peripheral nerve blocks.

Therefore, we conclude that the assessment of tissue oxygen saturation by NIRS in the evaluation of peripheral nerve block is a rapid, efficient, and practical method. We believe that as the first study in this field, our results would shed light on future studies which will investigate the role of the assessment of tissue oxygen saturation by NIRS in predicting the success of various peripheral nerve blocks.