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Anesthetic Pharmacology
Anesthesia and Pain Medicine 2011;6(3):225-230.
Published online July 30, 2011.
Effect of propofol on oxidative stress-attenuated glutamate transporter EAAT4 activity
Jung Hee Ryu, Chong Soo Kim, Sang Hwan Do, Hong Sik Park
1Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, Seongnam, Korea. cskim@brm.co.kr
2Department of Anesthesiology and Pain Medicine, Seoul National University College of Medicine, Korea.
3Department of Anesthesiology and Pain Medicine, Boramae Medical Center, Korea.
4Department of Anesthesiology and Pain Medicine, Seoul National University Hospital, Seoul, Korea.
Abstract
BACKGROUND
Propofol (2, 6-diisopropylphenol) has been known to have neuroprotective effects. Excitatory amino acid transporter 4 (EAAT4) is a glutamate transporter predominantly expressed in the cerebellar Purkinje cells, which is vulnerable to ischemic injury. Thus, we hypothesized that propofol reverses reduced EAAT4 activity which was induced by oxidative stress and investigated the effects of propofol on EAAT4 under oxidative stress induced by tert-butyl hydroperoside (t-BHP).
METHODS
EAAT4 was expressed in Xenopus oocytes by injection of its mRNA. By using two-electrode voltage clamping, membrane currents were recorded before, during, and after application of L-aspartate (3 microM) in the presence or absence of t-BHP and propofol.
RESULTS
L-aspartate induced an inward current in EAAT4 expressing oocytes. Exposure of these oocytes to t-BHP (1-20 mM) for 10 min dose-dependently decreased EAAT4 activity (1 +/- 0.01 microC for control; 0.88 +/- 0.05 microC for 1 mM; 0.83 +/- 0.03 microC for 2mM; 0.65 +/- 0.04 microC for 3 mM; 0.51 +/- 0.07 microC for 5 mM; 0.45 +/- 0.03 f microC for 10 mM and 0.24 +/- 0.06 microC for 20 mM). IC50 for t-BTH was 6.05 mM and further study was performed with 10 mM t-BTH. Propofol (3-10 microM) dose-dependently reversed this t-BHP-attenuated EAAT4 activity.
CONCLUSIONS
Oxidative stress by t-BHP decreased EAAT4 activity and 3-10 microM propofol restored oxidative stress-reduced EAAT4 activity.
Key Words: Excitatory amino acid transporter 4, Glutamate transporter, Propofol, Tert-butyl hydroperoside, Xenopus oocytes
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